For fully automated computer-assisted sperm analysis (CASA) systems, the selection of disposable counting chamber slides must meet requirements for high precision, standardization, contamination-free operation, and compatibility with automated analysis. Based on existing data, the following recommendations are provided:

Core Material and Parameter Requirements

1.  Counting chamber depth: 10 micrometers (0.01mm) is the industry standard. This depth ensures sperm form a single layer, preventing overlapping and accumulation. It positions sperm within the same focal plane under the microscope, enabling clear, stable imaging—the foundation for CASA systems to perform automatic identification and trajectory tracking.

2. Material: Specialized materials with high cleanliness, non-toxicity, and optical transparency should be used. While publicly available information does not explicitly specify glass types (e.g., borosilicate), it emphasizes “high cleanliness” and “non-toxicity.” This typically implies the use of specially treated premium glass or high-molecular-weight polymers to ensure sample stability and prevent impurities from interfering with imaging.

3. Design Type: Disposable, auto-aspirating designs are preferred. This eliminates cleaning requirements, completely prevents cross-contamination, and meets clinical and research hygiene standards. Its optimized venting structure ensures semen automatically and uniformly fills the counting chamber without manual smearing, reducing human error while improving throughput and repeatability.

4. Grid Structure: CASA-specific counting chambers typically do not require traditional grids. The depth and volume of the counting chamber are precisely calibrated. Combined with computer image analysis technology, it enables direct counting and motility analysis of a single layer of sperm, eliminating reliance on grids for manual estimation.

5. Compatibility: Counting chambers must comply with international CASA standards and OIE standards. This ensures their physical parameters (e.g., depth, volume) match the algorithms of the analysis software, guaranteeing the accuracy and comparability of test results.

Thus Fully automated computer-assisted sperm analysis systems should select dedicated counting chamber slides with a depth of 10 micrometers, single-use design, high-purity non-toxic materials, automatic aspiration functionality, and compliance with international CASA standards. Products specifically engineered for automated analysis (such as the Aisins CASA10 series mentioned in the documentation) maximize analytical accuracy, efficiency, and hygienic safety.

What is best disposable casa software system sperm counting chamber slide for computer assisted sperm semen analysis software system? I believe you definetely will give me answers like IMV LEJA Chamber Slide,Minitube Slide etc....But do you know which China brand CASA LEJA Chamber Slide is best?

AI SHEN IS China brand Slide Match Suitable Popular CASA System，the Must-Have Slide For CASA (Computer Assisted Sperm/Semen Analysis) To get the most from CASA Analyzer Systems (such as Hamilton Thorne IVOS II CASA Sperm Analyzer, CEROS II Microscope Sperm Analyzer, TOX IVOS II，Minitube AndroVision CASA Analyzer，MICROPTIC CASA Systems SCA System，IMV Technologies etc....), it is vital to use calibrated slides . Only calibrated slides provide the accuracy and precision needed to reliably analyze semen. But not all calibrated slides onmarket are ofthe same quality due to theirslide glass grade and coatingmaterial choosing.

Our  Slide is more accurate and more reliable than its competitors.And, it is a perfect tool to use with most CASA systems. It allows for the standardization of analyses for repeatable, objective measurements. Thisiswhy itiswidely used by semen production centers, veterinarians,and research centers. High technology and accuracy Semen analysis accuracy is crucial because it has a direct impact on the results calculated by CASA systems. This, in turn, may affect economic returns. Constant chamber depth A calibrated slide’ s accuracy is related To the consistency of chamber depth.AISHENSI has developed a production method that ensures constant precision stable depth. AISHENSI is China Original Sperm Counting Slide Manufacturer. 

To achieve this level of quality,AISHENSI has established strict controls: - inspection and tracking of raw materials - toxicity checks - depth checks - 18-point visual inspection High- tech fir s t gr ade materials The high quality of the glass being used ensures a faithful image of sperm cells, a quality required for CASA analyses. Producing slides in a clean room minimizes the risk of dust being deposited on the slide, thereby preventing reading errors and making the analyses even more accurate. All elements used are non-spermicidal, and a semen toxicity check is performed on every batch of slides produced. A certificate of analysis is issued for each batch.

Controlled filling of the AISHENSI slide chamber Filling the chamber smoothly is one of the most important steps to ensure for reliable concentration and sperm mobility measurements. To guarantee repeatability, the filling of the chambers with capillary action is made possible by our proprietary Special glass coating materials. Sperm do not stick to the slide, and are evenly distributed. Bubble formation is minimized and No Bubbles with our technology. These features allow standardized procedures and accurate measurements and may result in higher returns on investment for the user. Due to their special characteristics, AISHENSI slides both save time and increase the level of accuracy and precision for semen analysis. 

All slides are covered with a special coating to prevent air bubble formation and to prevent semen from sticking to the chamber surface. The resin and ink used are both non- toxic. Our  slides have an excellent low limit of quantification and can handle high sperm concentrations. The high level of accuracy and precision of AISHENSI  counting chambers will allow your results to be compared with other laboratories scientific data concerning the relationship between semen parameters ( concentration, motility, viscosity) and fertility. 

Usage: Using the micro pipette or pipette, it can add moderate concentration sample solution to the application area of the slide, and then the slide can automatically suck the sample into the chamber after the sample touch the edge of the cover slip.