What do you know about Ovine (sheep) and caprine (goat) sperm quality analysis?

Ovine (sheep) and caprine (goat) sperm quality analysis involves assessing volume, concentration, motility (movement), and morphology (shape) using microscopy and advanced techniques like Computer-Assisted Sperm Analysis (CASA) to check for issues like head defects or bent tails, ensuring high quality for breeding, especially for Artificial Insemination (AI), using standardized protocols and tests for plasma membrane integrity and DNA health.

What is the Key Parameters Assessed for casa sperm quality analysis software system:

Volume & Color: Milky white is ideal; clear or yellow can indicate dilution or contamination (urine).

Concentration: High sperm count (millions/mL) is crucial, with higher numbers needed for AI than natural service.

Motility: Evaluates progressive movement (forward motion) and wave motion (swirling) under a heated microscope (37°C).

Morphology: Microscopic check for normal head, midpiece, and tail; abnormalities like bent tails are indicators of poor quality.

Viability/Integrity: Assessed with fluorescent stains (e.g., for membrane integrity, mitochondrial function, DNA damage).

 Acrosome Integrity: Checks the cap over the sperm head, essential for fertilization.

Advanced Techniques:

CASA Systems: Standardize analysis, quantify motility, velocity, and perform basic morphology and advanced functional tests (hyperactivation).

Flow Cytometry: Uses fluorescent probes for detailed cell analysis (viability, DNA integrity, etc.).

Important Considerations:

Temperature Control: Maintaining 37°C during examination is vital for motility.

Sample Handling: Avoid contamination and rough handling; collect from the sperm-rich fraction.

Time Sensitivity: Motility and pH should often be checked within minutes of collection.

AI Suitability: High motility (e.g., >60%) and low abnormality (e.g., <4% abnormal heads) are targets, though specific thresholds vary.

For computer assisted sperm analysis software system,we must use perfect quality disposable sperm counting leja chamber slide to make sure the analysis precision.Our AISHENSI disposable sperm counting chamber slide perfect alternative to IMV LEJA Slide and Minitube Slide

Do you know the differences Between Makler Sperm Counting Slides and Disposable Sperm Counting Slides?

The core distinction between these two sperm counting slides lies in their design purpose and application scenarios: Makler slides are universal, suitable for both manual and automated analysis; disposable sperm counting slides (such as IMV LEJA SLIDE) are specifically optimized for automated analysis systems, offering higher precision and minimizing contamination.

1. Makler Sperm Counting Chamber

Structure: Slide + grid-patterned coverslip, well depth 10 micrometers.

Features: Grid etched onto coverslip; no semen dilution required; supports manual or automated counting.

Applications: Manual microscopic observation or basic automated analysis.

2. Disposable Sperm Counting Slides (e.g., LEJA Slide)

Structure: Slide + hydrophobic layer + cover slip, well depth 20 micrometers.

Features: Gridless design, capillary action sample loading, specifically engineered for automated analysis systems.

Applications: Automated semen analyzers, prevents cross-contamination.

In short, if you're using a high-end automated analyzer, choose disposable sperm counting slides; for manual or basic equipment, Makler slides offer greater cost efficiency.